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Search for "lipo-oligonucleotide duplexes" in Full Text gives 1 result(s) in Beilstein Journal of Organic Chemistry.

Specific DNA duplex formation at an artificial lipid bilayer: fluorescence microscopy after Sybr Green I staining

Emma Werz and
Helmut Rosemeyer

Beilstein J. Org. Chem. 2014, 10, 2307–2321, doi:10.3762/bjoc.10.240

complexes consisting of various DNA duplexes and the fluorescent dye were studied with respect to the kinetics of their formation as well as to their stability against perfusion. Keywords: artificial lipid bilayers; lipo-oligonucleotide duplexes; nucleic acids; Sybr Green I; Introduction The post

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Graphical Abstract

Figure 1: Chemical formulae and lipo-oligonucleotide sequences.

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Figure 2: Schematic illustrations (experiments A–F) of the specific DNA duplex formation at artificial lipid ...

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Figure 3: Experimental setup. Schematic drawing of the laser scanning microscope, the optical transparent mic...

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Figure 4: Chronological protocol of duplex formation of the lipo-oligonucleotide 4 with the complementary Cy5...

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Figure 5: Comparison of the bilayer brightness intensity with either Cy5 (irradiation: 635 nm) or Sybr Green ...

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Figure 6: Scheme of a z-scan of a lipid bilayer showing two locations for measurements of the diffusion times...

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Figure 7: Experiment B (4 + 6 + SG). Bilayer brightness as function of the various events (addition of oligon...

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Figure 8: Experiment D (4 + 9 + SG). Bilayer brightness as function of time and the various events [addition ...

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Figure 9: Conceivable geometry of the complex at the bilayer surface (cis compartment).

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Figure 10: Experiment E (10 + 6 + SG). Bilayer brightness as a function of time as well as of various events [...

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Figure 11: Experiment E (10 + 6 + SG). Bilayer brightness as function of the incubation time (A), of the perfu...

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Figure 12: Z-scans of the experiment E before and after the addition of Sybr Green I. A) Z-scan after the addi...

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Figure 13: Experiment E in reversed order of component addition; SG + 10 + 6.

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Figure 14: Bilayer brightness as a function of perfusion number and incubation periods for the experiment E in...

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Figure 15: Bilayer brightness as a function of perfusion number and incubation periods for the experiment F (SG...

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Figure 16: Kinetics of the tertiary complex formations of various lipo-DNA/DNA with Sybr Green I during incuba...

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Figure 17: Trafficking of a siRNA by a lipophilized DNA.

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Figure 18: A) Stage unit of the ‘Ionovation Explorer’ mounted on a standard inverted fluorescence microscope. ...

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Published 02 Oct 2014

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